Abstract
Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain R. kratochvilovae CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans.
Highlights
Faculty of Science and Technology, Norwegian University of Life Sciences, P.O
CCY 20-2-26 grew in the presence of various nitrogen sources, such as potassium nitrate, yeast extract, ammonium sulfate, ammonium chloride and urea
Yeast extract and urea appear to be the most effective for the growth of R. kratochvilovae CCY 20-2-26, where the yields reached a value of about 9.0 g/L of biomass
Summary
Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech Republic; Abstract: Multifunctional biomass is able to provide more than one valuable product, and it is attractive in the field of microbial biotechnology due to its economic feasibility. Multifunctional biomass, i.e., the possibility of isolation of a broad spectrum of metabolites from one single fermentation process, is the perspective task that establishes a costeffective microbial production and ensures the economic feasibility of microorganism cultures. Yeast), in which the genus of Rhodotorula is the most studied This yeast can utilize a wide range of substrates, mono- and polysaccharides and waste materials while producing highvalue substances [6]. Besides carotenoids, these unicellular microorganisms are capable of producing a variety of biotechnologically important metabolites, such as lipids [7], ergosterol [8], enzymes [9], extracellular molecules [10] and polysaccharides [11]
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