Rhodobacter capsulatus Gene Transfer Agent Transduction Assay

Abstract

The gene transfer agent (GTA) is a bacteriophage-like particle that transfers genomic DNA from a donor to a recipient bacterium. The Rhodobacter capsulatus GTA (RcGTA) was the first to be studied and this protocol has been optimized for RcGTA transduction, although it could be modified for other bacteria containing a GTA. The RcGTA transduction assay can be used to determine transduction efficiencies, to create gene knock-outs, or to create new strains by transferring alleles from one strain to another., 基因转移剂（GTA）是将基因组DNA从供体转移到受体细菌的噬菌体样颗粒。 红细菌（Rhodobacter capsulatus）GTA（RcGTA）是第一个被研究的，并且该方案已经针对RcGTA转导进行了优化，尽管其可以对于包含GTA的其他细菌进行修饰。 RcGTA转导测定可用于测定转导效率，产生基因敲除，或通过将等位基因从一个菌株转移到另一个菌株来产生新菌株。