Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function.

Delphine Gendre,Thomas Stanislas,Nicolas Esnay,Stéphane Claverol,Rishikesh P Bhalerao,Markus Grebe,Anirban Baral,Yohann Boutté,Anna Gustavsson,Thomas Vain,Xie Dang,Deshu Lin

doi:10.1242/dev.168559

Abstract

ABSTRACTRoot hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.

Highlights

Root hair formation in plants underlies strict spatial control and in Arabidopsis thaliana (Arabidopsis) root hairs emerge from the basal ends of hair-forming epidermal cells
Our analyses of yip4a yip4b double mutant roots indicated an almost complete absence of root hairs compared with wild type (WT) (Fig. 1A and Fig. S1A,B), with rare or no visible bulges, whereas the single yip4a and yip4b mutants have a similar or slightly higher hair density than wild type, respectively (Fig. S1A,B)
Mutations that result in a failure to specify trichoblast identity cause the formation of fewer or no hairs and ectopic hair cell specification results in additional hairs

Summary

Introduction

Root hair formation in plants underlies strict spatial control and in Arabidopsis thaliana (Arabidopsis) root hairs emerge from the basal (root tip-oriented) ends of hair-forming epidermal cells. As the absence of visible bulges in yip4a yip4b indicated that YIP4s may act at an early stage of root hair development, we investigated the recruitment of ROPs to the basal end of trichoblasts, preceding the formation of the bulge (Molendijk et al, 2001; Jones et al, 2002).

Results

Conclusion