Rhizospheric soil organic carbon accumulated but its molecular groups redistributed via rhizospheric soil microorganisms along multi-root Cerasus humilis plantation chronosequence at the karst rocky desertification control area.

Abstract

Though the relationships between the microorganism communities and the edaphic factors in rhizosphere soil along the plantation chronosequence have been widely reported, few researches have appeared on the interrelationship about rhizospheric soil microorganism community and soil organic carbon (SOC) under multi-root Cerasus humilis plantations of different age. In our study, the rhizospheric soil microbial communities, soil physicochemistry, and SOC molecular groups in plantations of 1-, 3-, and 5-year-old Cerasus humilis were investigated in karst rocky desertification control area of southwest China. It was found that karst rhizospheric soil moisture, total nitrogen, available potassium, and 46-60ppm N-alkyl/methoxyl C decreased; however, SOC and fungal:bacterial ratio decreased along multi-root Cerasus humilis plantation chronosequence. Proteobacteria, Actinobacteriota, Acidobacteriota, and Ascomycota were recognized as the top 4 phyla in the karst rhizospheric soil microbial co-occurrence network. Moreover, Cerasus humilis plantations exerted significantly direct effect on rhizospheric soil microbial communities and soil physicochemical properties exerted significantly direct effects on SOC molecular groups. Our results suggested that the increased Cerasus humilis plantation years will promote C sequestration (e.g., SOC) with the continued input of root litter, root exudates, and plant litter. The inputted and activated C can be preferentially consumed by rhizospheric soil microorganisms and converted into microbial-derived compounds, which are finally incorporated into recalcitrant SOC pools. Hence, Cerasus humilis redistributed SOC molecular groups via rhizospheric soil microorganisms, and increased ratio of fungi:bacteria in rhizosphere was associated with C sequestration which could not be regarded as a widespread rule. Though our study is the first attempt to recognize the interaction between rhizospheric soil microbial community and SOC molecular groups at the karst rocky desertification control area, it provides a baseline for further research that ecological restoration can promote soil C sequestration via soil microorganisms in the early period of eco-restoration at karst area.