Rhizosphere effect and its associated soil-microbe interactions drive iron fraction dynamics in tidal wetland soils

Abstract

It is becoming increasingly clear that plants can affect iron (Fe) dynamics in tidal wetland soils, but whether this is rhizosphere effect-dependent remains unclear. To assess rhizosphere effects on soil Fe cycling, in-situ rhizosphere and bulk soil samples (0–60-cm) were collected from a tidal wetland across plant growth stages (regreening, shooting, and senescence). Changes in Fe fractions, the abundance of Fe-oxidizing/reducing bacteria (16S rRNA gene), root morphology traits, and soil and porewater geochemistry were examined. Overall, the rhizosphere effect decreased soil pH but increased the concentrations of dissolved organic carbon (DOC), porewater Fe2+, and bicarbonates (HCO3−). Both Fe-oxidizing and Fe-reducing bacteria were more enriched in the rhizosphere than those in the bulk soil. The rhizosphere effect increased the concentrations of amorphous and crystalline Fe(III), and also enhanced the proportion of amorphous Fe(III). The rhizosphere had higher concentrations of non-sulfidic ferrous iron [Fe(II)] but lower concentrations of ferrous sulfide (FeS) and pyrites (FeS2) than those in bulk soils, suggesting that the rhizosphere effect favors microbial Fe(III) reduction but suppresses microbial sulfate reduction. Moreover, the rhizosphere amorphous Fe(III) levels changed following the patterns of root porosity, which attained peak values at the root tips. The abundance of Fe-reducing bacteria was controlled by both DOC and amorphous Fe(III) concentrations, which were relatively higher during the regreening and shooting stages than those during the senescence stage. Taken together, our findings highlight that the rhizosphere effect transfer Fe from the bulk soil to the rhizosphere and especially redirects it from FeS associations to microbially-mediated Fe redox cycling. This rapid Fe redox cycling could be responsible for buffering soils and organisms from sulfide accumulation and stimulate C mineralization in the tidal wetland ecosystem.