Abstract
Melia azedarach-rhizosphere mediated degradation of benzo(a)pyrene (BaP), in the presence of cadmium (Cd) was studied, using efficient rhizobacterial isolate. Serratia marcescens S2I7, isolated from the petroleum-contaminated site, was able to tolerate up to 3.25 mM Cd. In the presence of Cd, the isolate S2I7 exhibited an increase in the activity of stress-responsive enzyme, glutathione-S-transferase. Gas Chromatography-Mass spectroscopy analysis revealed up to 59% in -vitro degradation of BaP after 21 days, while in the presence of Cd, the degradation was decreased by 14%. The bacterial isolate showed excellent plant growth-promoting attributes and could enhance the growth of host plant in Cd contaminated soil. The 52,41,555 bp genome of isolate S. marcescens S2I7 was sequenced, assembled and annotated into 4694 genes. Among these, 89 genes were identified for the metabolism of aromatic compounds and 172 genes for metal resistance, including the efflux pump system. A 2 MB segment of the genome was identified to contain operons for protocatechuate degradation, catechol degradation, benzoate degradation, and an IclR type regulatory protein pcaR, reported to be involved in the regulation of protocatechuate degradation. A pot trial was performed to validate the ability of S2I7 for rhizodegradation of BaP when applied through Melia azedarach rhizosphere. The rhizodegradation of BaP was significantly higher when augmented with S2I7 (85%) than degradation in bulk soil (68%), but decreased in the presence of Cd (71%).
Highlights
Melia azedarach-rhizosphere mediated degradation of benzo(a)pyrene (BaP), in the presence of cadmium (Cd) was studied, using efficient rhizobacterial isolate
Serratia marcescens S2I7, isolated from petroleum-contaminated soil was found to be highly resistant to a higher concentration of cadmium and have plant growth promotion (PGP) traits, and could degrade BaP efficiently
Considering these, the present work was undertaken to study the genome of S. marcescens S2I7, a Cd-tolerant bacterium with PGP properties that can efficiently degrade polycyclic aromatic hydrocarbons (PAHs)
Summary
Melia azedarach-rhizosphere mediated degradation of benzo(a)pyrene (BaP), in the presence of cadmium (Cd) was studied, using efficient rhizobacterial isolate. Several isolates were screened for unique abilities to degrade benzo(a)pyrene (BaP), in addition to resistance for Cd and have plant growth promotion (PGP) attributes too. Serratia marcescens S2I7, isolated from petroleum-contaminated soil was found to be highly resistant to a higher concentration of cadmium and have PGP traits, and could degrade BaP efficiently. Considering these, the present work was undertaken to study the genome of S. marcescens S2I7, a Cd-tolerant bacterium with PGP properties that can efficiently degrade PAHs. To evaluate the potential of this isolate for bioremediation of hydrocarbon contamination sites, its genome was studied, along with analytical analysis of PAH degradation in-vitro conditions and with the association of Melia azedarach plant
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