Rhizosphere activity induced mobilization of heavy metals immobilized by combined amendments in a typical lead/zinc smelter-contaminated soil

Abstract

The persistence of the stabilization effect of amendments on heavy metals (HMs) is of great concern when they are used for remediating HM-contaminated soil. Here, pot experiments were conducted to investigate the effects of two consecutive seasons of vegetable cultivation on the mobilization of HMs (Cu, Pb, Zn, and Cd) immobilized by different application ratios (0, 20, 40, and 80 g kg−1, labelled C0, C2, C4, and C8) of a combined amendments (lime: sepiolite: biochar: humic acid = 2:2:1:1). The results showed that HM bioavailability decreased with increasing application ratios of the combined amendments in control (CK) treatments. The DOC contents, HM bioavailability, and HM contents in the leaves of vegetables increased, but the pH decreased during two consecutive seasons of vegetable cultivation; however, the HM bioavailability in the C2, C4, and C8 treatments was lower than that in the C0 treatments with vegetables. Catalase, urease, alkaline phosphatase, and dehydrogenase activities in the combined amendment treatments with and without vegetables were decreased compared to those in the C0 treatments. The relative abundances of the dominant bacterial phyla in the different treatments were Actinobacteria > Proteobacteria > Chloroflexi > Acidobacteria > Gemmatimonadetes > Bacteroidetes for the first season and Proteobacteria > Actinobacteria > Chloroflexi > Acidobacteria > Bacteroidetes > Gemmatimonadetes for the second season. Correlations showed that the pH and DOM properties during two consecutive seasons of vegetable cultivation were important factors influencing HM bioavailability, enzyme activity, and bacterial community composition. The bacterial community composition shift indirectly influenced the mobilization of HMs immobilized by the combined amendments. Thus, rhizosphere activity induced the mobilization of HMs immobilized by combined amendments during two consecutive seasons of vegetable cultivation.