Abstract
Rhizopus oryzae BD5, which produced lipase to catalyze transesterification of soybean oil and alcohol to form fatty acid alkyl ester (so-called biodiesel) and glycol, was isolated from petroleum hydrocarbon-contaminated soils by selection method. The strain was cultured in optimal medium included nonwoven fibers using a composition with yeast extract 0.5% (w/w), pharmamedia 0.4% (w/w) and olive oil 0.2% (w/w) for intercellular lipase production. After 72 h fermentation, the R. oryzae BD5 immobilized cell was collected to transesterificate with various operating conditions. The optimal conditions for fatty acid alkyl esters production by transesterification with the immobilized cell were a 1-butanol/soybean oil molar ratio 3: 1, immobilized cell concentration 10 wt.% and minimum of 10 wt.% added water. The 65.2% conversion rate of fatty acid alkyl esters was obtained in 24 h at 30℃.
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