Abstract
We isolated and identified a gibberellin-producing Burkholderia sp. KCTC 11096 from agricultural field soils. The culture filtrate of plant growth promoting rhizobacteria (PGPR) significantly increased the germination and growth of lettuce and Chinese cabbage seeds. The ethyl acetate extract of the PGPR culture showed significantly higher rate of lettuce seed germination and growth as compared to the distilled water treated control. The ethyl acetate fraction of the Burkholderia sp. was subjected to bioassay-guided isolation and we obtained for the first time from a Burkholderia sp. the plant growth promoting compound rhizonin A (1), which was characterized through NMR and MS techniques. Application of various concentrations of 1 significantly promoted the lettuce seed germination as compared to control.
Highlights
Secondary metabolites have mainly been isolated and characterized from microbes for industrial and medicinal purposes [1,2]
It has been shown that the: (1) secondary metabolite synthesis by rhizobacteria may correspond to its respective taxon and ecological niche while (2) metabolic interactions may enhance the synthesis of several metabolites as well as (3) effect plant growth and development [3,4]
The culture filtrate (CF) of Burkholderia sp. was applied to lettuce and Chinese cabbage seeds which were incubated for three days at room temperature in the dark
Summary
Secondary metabolites have mainly been isolated and characterized from microbes for industrial and medicinal purposes [1,2]. For the microbiologist studying ecology, it is apparent that secondary metabolites play a role in vivo and are important for various metabolic interactions between microorganisms and their plant hosts. It has been shown that the: (1) secondary metabolite synthesis by rhizobacteria may correspond to its respective taxon and ecological niche while (2) metabolic interactions may enhance the synthesis of several metabolites as well as (3) effect plant growth and development [3,4]. Rhizobacteria are one of such sources which can be used for intelligent screening of bioactive metabolites. The isolated metabolites of bacteria belong to diverse classes of compounds including steroids, xanthones, phenols, isocoumarins, perylene derivatives, quinones, furandiones, terpenoids, depsipeptides, and cytochalasines [5]. The biotechnological use of these metabolites for pharmaceutical or agrochemical products is still in the developmental stage
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