台灣水稻紋枯病病原菌Rhizoctonia solani AG1-IA菌株之多樣性

Abstract

Rice is used to be the dominated food in many countries, with the suffering of plant pathogen like rice blast and rice sheath blight, the production is reduced. Rice sheath blight caused by the pathogen of Rhizoctonia solani AG1-IA and the pathogen dominantly happens in the area with high temperature and moist weather. Besides, the weather above each season with humidity and high temperature facilitate the serious disease. Therefore, with the understanding of the genetic difference, characteristic, and the difference of disease severity of different test isolates, the process to discriminate the similarity and the resource of each test isolates collected from, to have the idea of condition of rice sheath blight in Taiwan could became possible. Besides, after the recognition of anastomosis test and the specific primer Rho1 and Rho2 designed to detect the specific sequence of test isolates, the result of molecule tool totally similar with the anastomosis test. That means the molecule tool can separate the difference between different groups of Rhizoctonia solani. Besides, with the composeion of the information with RAPD of 13 primers, the cluster of discrimination with phylogenetic tree of each test isolates and reference isolates are recognized in to 8 clusters at the simileruty of 48%. The clusters with phylogenetic tree of dsRNA are divided into also 8 clusters at similarity of 72%. On the otherhand, with the calculation of diseasivity after the inoculation with rice culture in greenhouse, the disease severity is subsequently worse form Tai-nung No. 71 to Tai-keng No.9 and to Tai-keng No. 11. Besides, there is no relativity of the elements of the quantity, hypha growth rate and the radius of the distribution of sclerotium of test isolates pretend to be positive related. And the existing of dsRNA seems to have nothing positively related with the disease severity of inoculation of test isolates and the phenomenon with the culture of test isolates. The conclusion is that the genetic characteristic of test isolates are related with the geographical resource of water irrigation is the valuable result of this research.