Abstract
The objective of this study was to (1) identify the causal agent of damping-off observed on Garnem (Prunus amygdalus x Prunus persica) rootstock micropropagated plants during acclimatization phase; and (2) test the potential antagonism of Rhizobia against this dangerous fungus. In addition to the previously published results (KY964496, KY964499), further isolates have been collected and characterized. Morphological traits of these isolates and sequencing of their rDNA ITS gene showed that they also belong to Rhizoctonia solani anastomosis group 1-IC (AG-1-IC). Two supplementary sequences were deposited in GenBank as KY964497 and KY964498. Pathogenicity testing confirmed the direct implication of this pathogen in wilting, crown and root rot symptoms that may lead to plant damping-off. The possibility to control R. solani by using Rhizobia as a potential antagonist has been further investigated. In vitro dual culture of R. solani and 40 Rhizobium strains resulted in different mycelium inhibition (I) rates. The significantly highest I rates were observed with strains from pea (PP6, PPOUEDP292, PP29), chickpea (Pch Kass) and bean (HaD4002). In vivo test showed that HaD4002 (T4) conferred the highest survival rate (21%).
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