Abstract
The threat of arsenic (As) pollution has become serious and leading to opt of low-cost microbial remediation strategies.Some bacteria have the ability to resist As. A group of rhizosphere bacteria have the ability to absorb arsenic. So these bacteria may be a good candidate for arsenic bioremediation from contaminated environment. Our present study of identifying suitable rhizobacterial strains led to the isolation of As-tolerant strains from arsenic pollutedrhizospheric soils of lentil in West Bengal, India.The isolated rhizobacterial strain LAR-7 had a high MIC (minimum inhibitory concentration) towards arsenate (260 mM) and arsenite (27.5 mM) and transformed 39% of arenite to arsenate under laboratory condition. Further, the strain LAR-7 had enormous plant growth-promoting characteristics (PGP), as categorized by efficient ability to solubilize phosphate, siderophore production, production of indole acetic acid-like molecules, ACC deaminase production, and nodule formation under As stressed condition. Based on 16S rRNA homology the LAR-7 was identified as Rhizobium leguminosarum andemerged as the most potent strain for As decontamination and plant growth promoter under the stress environment of As.
Highlights
The human populations of eastern India (West Bengal mainly) and Bangladesh are severely suffered by arsenic (As) contamination of water and food.[1, 2] The concurrent application of As enriched irrigation seems to be the major reason for soilChandra Krishi Viswavidyalaya, Mohanpur 741252, Nadia, West Bengal, India.LAHA et al, Curr
A thin layer of bacterial cells were kept on a glass cover slip and prepared a smear, after that it was allowed to heatfix over a flame for 1–2 sec followed by fixation with 2.5% glutaraldehyde for 45 min.[30]
Arsenite Transformation and Species Detection The arsenite transformation ability of the bacterial isolate was determined by modified laboratorybased standard protocol.[27]
Summary
The human populations of eastern India (West Bengal mainly) and Bangladesh are severely suffered by arsenic (As) contamination of water and food.[1, 2] The concurrent application of As enriched irrigation seems to be the major reason for soilChandra Krishi Viswavidyalaya, Mohanpur 741252, Nadia, West Bengal, India.LAHA et al, Curr. Build-up,[3, 4] and subsequent accumulation in standing crops.[5] Generally, As residues are found in the top layer of soil or surface soil and enter the plants because of their low volatility and low solubility.[6] As has both organic and inorganic forms[7] that are found as an oxyanion in the environment.[8] The high-risk involvement and high cost of remediation[9] emphasize the scope of bioremediation through novel As-tolerant microbes.[10] aseptic condition from arsenic (As) contaminated rhizospheric zone of lentil in Chakdaha, West Bengal (23o05' N latitude and 88o54' E longitude), India, noted for As concentrations in the groundwater above World Health Organization (WHO)-defined safe limit.[22] Atomic Absorption Spectrophotometer (AAS, Model-Perkin Elmer A Analyst 200) was used to measure total[23] and available As24 of the soil samples
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