Rhizobacterial Bacillus mycoides functions in stimulating the antioxidant defence system and multiple phytohormone signalling pathways to regulate plant growth and stress tolerance.

Abstract

To analyse effects and mechanisms of plant growth promotion mediated by Bacillus mycoides strain A3 (BmA3), in Arabidopsis thaliana seedlings. Bacillus mycoides strain A3 (BmA3) isolated from the bamboo rhizosphere produced phytohormones, including indole-3-acetic acid (IAA) and gibberellic acid (GA), and exhibited phosphate solubilization and radical scavenging activities. A. thaliana seedlings inoculated with BmA3 exhibited an altered root architecture including an increased number of lateral roots and root hairs. Likewise, enhanced photosynthetic efficiency through the accumulation of higher levels of chlorophyll and starch, and increased plant size and fresh weight were observed in the BmA3-treated seedlings. This bacterial inoculation stimulated the antioxidant defence system by increasing the activities of catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and phenylalanine ammonia-lyase (PAL). Secondary metabolites, including phenolic compounds, flavonoids and glucosinolates, were induced to higher levels in the BmA3-treated plants. Under drought and heat stresses, lower levels of H2 O2 , malondialdehyde (MDA) and electrolyte leakage were noticed in the treated seedlings. Genes involved in the signalling pathway of jasmonic acid (JA) including MYC2 and lipoxygenase 1 (LOX1) and salicylic acid (SA) including SAR DEFICIENT 1 (SARD1) and CAM-BINDING PROTEIN 60-LIKE G (CBP60G), and the antioxidant defence system including Ascorbate peroxidase (AtAPX) and alternative oxidase (AOX) were upregulated in BmA3-treated plants. Moreover, pathogenesis-related protein 1 (PR-1) and PR-2, marker genes for disease resistance, as well as DREB2A and HsFA2, which function in abiotic stress regulation, were also upregulated. BmA3 was able to activate JA and SA signalling pathways to induce plant growth and abiotic stress tolerance in A. thaliana seedlings. The plant growth promotion and increased stress tolerance induced by BmA3 were the result of the combined effects of microbial metabolites and activated host plant responses, including phytohormone signalling pathways and antioxidant defence systems.