Abstract
We isolated a rhesus monkey rhadinovirus, isolate RRVmmu 209-07, from hemangioma tissue. The virion DNA was sequenced by Illumina-based sequencing. Isolate RRVmmu 209-07 is highly similar overall to RRV 26-95, but considerable differences exist in the 3' region of the genome.
Highlights
A male rhesus macaque experimentally infected with simian-human immunodeficiency virus for 559 days at the New England Regional Primate Research Center (1) presented at study endpoint with hemangiomas in the mandibular gingiva, lower abdomen, and scrotum
384,400 paired reads of 150 nucleotides and 1,067,294 paired reads of 250 and 50 nucleotides were obtained on the Illumina MiSeq system using the MiSeq reagent kit (300 cycle)
A BLAST (3) search with individual contigs demonstrated the presence of a rhesus monkey rhadinovirus (RRV; order Herpesvirales, family Herpesviridae, subfamily Gammaherpesvirinae)
Summary
A male rhesus macaque (animal 209-07) experimentally infected with simian-human immunodeficiency virus for 559 days at the New England Regional Primate Research Center (1) presented at study endpoint with hemangiomas in the mandibular gingiva, lower abdomen, and scrotum. DNase-protected DNA was used to prepare a double-indexed Illumina-compatible library using the Nextera DNA sample preparation system (Illumina, San Diego, USA). 384,400 paired reads of 150 nucleotides and 1,067,294 paired reads of 250 and 50 nucleotides were obtained on the Illumina MiSeq system using the MiSeq reagent kit (300 cycle).
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