Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation

Jin-Run Dong,Li-Yun Guo,Ren-Li Qi,Chun-Jie Xiao,Jia-Gui Qu,Wen-Chao Wang,Zheng-Bo Wang

doi:10.3724/sp.j.1141.2012.01043

Abstract

To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12(th) days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.

Highlights

To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established
More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12th days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain
The rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future

Summary

Introduction

To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation The rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future. 碱性成纤维细胞生长因子 (basic fibroblast growth factor, bFGF) 是 Gospodarowicz (1975) 在 1974 年从牛脑中分离纯化出的一种多肽因子。 bFGF 在胚脑及成年脑中均有分泌, 是一种重要的有丝分裂原, 它能通过作用于细胞表面相应的受体促进神经干细胞的增殖和分化。表皮生长因子 (epid-ermal growth factor, EGF)是 Cohen(1962)首次在小鼠的颌下腺中发现的一种小分子蛋白, 也具有广泛的促丝裂增殖的作用。当前普遍采用 bFGF 和 EGF 进行扩增培养神经干细胞(Murphy et al, 1990; Studer et al, 1998), 而 bFGF/EGF 扩增是否影响干细胞特性存在争议 (Du & Zhang, 2004; Elkabetz & Studer 2008; Hong et al, 2008; Koch et al, 2009)。

Results

Conclusion