Abstract
In this study, we aim to reveal the properties of different fractions of soy hull soluble polysaccharide (SHSP) derived by alcohol precipitation and the related mechanism. Three fractions were obtained, designated as SHSP20, SHSP40 and SHSP60; every fraction has its advantage(s). The molecular weight, monosaccharide composition, functional groups, morphology, and other rheological properties of all SHSP fractions were investigated. The results showed that SHSP20 and SHSP40 accounted for 74.3% of soy hull soluble polysaccharide. FTIR spectrum confirmed the presence of uronic acid and protein in SHSP fractions. Also, the molecular weight of the polysaccharide fractions varied from 124.21 to 381.83 kDa. GC analysis indicated that mannose, galacturonic acid, and galactose were the main monosaccharide components of all the SHSP fractions. However, the monosaccharide types and ratios of the three SHSP fractions are significantly different. Furthermore, shear thinning behaviour of SHSPs solution was observed. SHSP40 exhibited the highest viscosity among samples tested. Atomic force microscopy further confirmed the various morphologies of SHSP fractions. The results suggest that SHSPs obtained by gradient alcohol precipitation have different molar masses and chain conformations.
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