Abstract
This study aimed to prepare and preliminarily characterize the potential bone restorative effect of composite hydrogels based on hydroxypropylmethylcellulose (HPMC), graphene oxide (GO), and polysaccharides from Agaricus blazei Murill mushroom (PAb). The chemical composition and morphology of the biomaterials were analyzed using FTIR, XRD, SEM, and EDS. Rheological tests were conducted on the hydrogels to assess their flow properties for in vivo application and product stability. Cytotoxicity assays were performed using the GO (1 – 100 µg mL–1) against human neutrophils. Additionally, an in vivo tooth extraction test was carried out by applying HPMC hydrogels containing GO and PAb directly into the alveoli to enhance oral bone restoration. The rheological behavior of the hydrogels followed the Ostwald de Waele model (R² > 0.99), with flow index (n) values below 1.0, indicating a pseudoplastic behavior. Furthermore, the consistency index (k) values increased with the addition of GO and PAb, suggesting a more complex structure, and altered flow behavior at lower shear rates. In the MTT assay, GO exhibited low cytotoxicity towards human neutrophils, maintaining cell viability above 95.0 % even at high concentrations. For the in vivo study, a bisphosphonate-induced osteonecrosis model was used to assess bone tissue healing through macroscopic and radiographic analyses. A significant improvement was observed in the reduction of bone tissue loss in rats treated with the hydrogels compared to the negative control (ZA group). These findings indicate that composite hydrogels containing HMPC/GO/PAb have the potential to be used as biomaterials for mitigating bone loss.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
