Abstract
Pseudomonas protegens strain BNJ-SS-45 possesses potential to secrete multiple antifungal molecules and other metabolites. To expand its metabolic potential, herein, an attempt has been made to characterize rhamnolipid and siderophore molecules. The analysis of purified biosurfactants using liquid chromatography-tandem mass spectrometry (LC–MS/MS) revealed different congeners where mono-rhamnolipids Rha-C10-C14:1/Rha-C12-C12:1, Rha-C10-C12/Rha-C12-C10, Rha-C12-C12/Rha- H2O C10-C14/Rha-C14-C10, Rha-C10-C10:1/Rha-C10:1-C10, Rha-C10-C12:1/Rha-C12:1-C10 are present. Structural investigation of XAD-4 purified siderophores using liquid chromatography quadrupole time-of-flight mass spectrometry (LC–MS-QTOF) analysis revealed the presence of isoquinoline N oxide as a chromophore. Using response surface methodology for optimization a sustainable increase in the level of metabolites is achieved and resulted in 81% and 66% enhancement in rhamnolipids and siderophores level in a 7 L fermentor respectively. Rhamnolipids obtained also exhibited antagonistic activity against both pathogenic fungi and bacteria. These are promising candidates to be used as biocontrol agents especially due to their biodegradable and eco-friendly nature.
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