Abstract
Herein, we reported for the first time that RGD-conjugated silica-coated gold nanorods on the surface of multiwalled carbon nanotubes were successfully used for targeted photoacoustic imaging of in vivo gastric cancer cells. A simple strategy was used to attach covalently silica-coated gold nanorods (sGNRs) onto the surface of multiwalled carbon nanotubes (MWNTs) to fabricate a hybrid nanostructure. The cross-linked reaction occurred through the combination of carboxyl groups on the MWNTs and the amino group on the surface of sGNRs modified with a silane coupling agent. RGD peptides were conjugated with the sGNR/MWNT nanostructure; resultant RGD-conjugated sGNR/MWNT probes were investigated for their influences on viability of MGC803 and GES-1 cells. The nude mice models loaded with gastric cancer cells were prepared, the RGD-conjugated sGNR/MWNT probes were injected into gastric cancer-bearing nude mice models via the tail vein, and the nude mice were observed by an optoacoustic imaging system. Results showed that RGD-conjugated sGNR/MWNT probes showed good water solubility and low cellular toxicity, could target in vivo gastric cancer cells, and obtained strong photoacoustic imaging in the nude model. RGD-conjugated sGNR/MWNT probes will own great potential in applications such as targeted photoacoustic imaging and photothermal therapy in the near future.
Highlights
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1,2,3]
Our results showed that RGD-Gold nanorods (GNRs)-multiwalled carbon nanotubes (MWNTs) probes will own great potential in applications such as targeted PA imaging and photothermal therapy in the near future
The well-distributed silica-coated gold nanorods (sGNRs) deposited onto the surface of MWNTs showed that the Carbon nanotubes (CNTs) pre-treatment was effective, which resulted in many active sites on the MWNTs
Summary
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1,2,3]. How to recognize and track or kill early gastric cancer cells is a great challenge for early diagnosis and therapy of patients with gastric cancer. We have tried to establish an early gastric cancer prewarning and diagnosis system since 2005 [5,6]. We hoped to find early gastric cancer cells in vivo by multimode targeted imaging and serum biomarker detection techniques [7,8,9,10,11,12]. Development of safe and highly effective nanoprobes for targeted imaging and simultaneous therapy of in vivo early gastric cancer cells has become our concern
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