RFLP Markers for the Selection of Pollination-constant and Non-astringent (PCNA)-Type Persimmon and Examination of the Inheritance Mode of the Markers

Abstract

We investigated marker-assisted selection of the pollination constant and non-astringent (PCNA) trait and inheritance modes of the marker locus in persimmon (Diospyros kaki Thunb.) using restriction fragment length polymorphism (RFLP) analysis. In two backcross progenies, FU-170 and FU-275, 5 of 71 and 23 of 101 offspring, respectively, were judged as PCNA genotype by the marker phenotype. The astringency type of individual offspring was determined by measuring the size of tannin cells and soluble tannin content in mature fruit, thus confirming complete co-segregation of RFLP markers and the non-PCNA phenotype in 32 and 68 fruit-obtainable offspring from FU-170 and FU-275, respectively; therefore, RFLP markers can be used for PCNA genotype detection. Their segregation ratio deviated significantly from that of disomic inheritance. We also discuss the possibility of polysomic inheritance of RFLP markers.