RFLP Mapping of QTLs Influencing Shoot Regeneration from Mature Seed‐Derived Calli in Rice

Abstract

This study focused on the mapping of quantitative trait loci (QTLs) related to shoot regeneration from mature seed‐derived calli of rice (Oryza sativa L.) by restriction fragment length polymorphism (RFLP) markers. The F2 population from a cross between ‘Norin 1’ (japonica) and ‘Tadukan’ (indica), which showed lower and higher shoot regeneration rates, respectively, was used for QTL interval mapping. The population was analyzed for 103 RFLP markers distributed over the 12 rice chromosomes. The QTL with the largest effect on shoot regeneration, which accounted for about 19% of the total variation, was mapped on chromosome 2. A minor QTL on chromosome 4 exhibited a smaller effect, accounting for about 3% of the total variation in percentage shoot regeneration. No QTLs were detected on other chromosomes with the RFLP markers that were used in the present experiments. These results indicate that at least one major gene is associated with the percentage shoot regeneration, and agree with previous genetic studies, indicating that one dominant gene is associated with shoot regeneration.