Abstract
This work presents an original approach to sense the lipid intracellular content of microalgae, by the Radio Frequency (RF) dielectric spectral characterization of these unicellular organisms. To do so, a “homemade” open-ended coaxial sensor was associated to a Vector Network Analyzer (VNA), which allowed a broadband characterization in the frequency range from 30MHz to 3GHz. The sensor combines a coaxial line with a modified SMA connector. This study shows that the lipid quantification can be determined with the complex dielectric spectra properties extracted from the reflection coefficient (S11) measurement. A calibration of the open-ended coaxial probe is performed by using three different conditions (vacuum, water, methanol) from which the complex dielectric behavior is mastered. A model inversion is finally achieved to estimate the complex permittivity spectrum of the biological samples in a large frequency range, which is related to the microalgae lipid content.
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