Reye's syndrome: Salicylates and mitochondrial functions

Abstract

The effects of aspirin (acetylsalicylate, ASA) and related compounds in the presence of Ca 2+ on the oxidative metabolism of isolated rat liver mitochondria were studied. Intact mitochondrial preparations preincubated with ASA + Ca 2+ exhibited a transient stimulation of the state 4 respiratory rate with NAD +-linked substrates, followed by an inhibition which could not be released by the addition of ADP or uncoupler. Maximum respiratory rates were achieved by subsequent addition of NAD + or succinate. The Ca 2+-transport inhibitors ruthenium red and ethylene glycol-bis-(β-aminoethyl ether) N, N′-tetraacetic acid (EGTA) prevented these effects. Five brands of commercial aspirin were tested and were as effective as purified ASA. Tylenol (acetaminophen) could reproduce these effects only at much higher (≥ 10-fold) concentrations. Other salicyl derivatives showed results qualitatively similar to ASA, with potencies in the order: acid ⋙ASA > alcohol ≥ catechol > amide, salicylate being approximately 10-fold more potent than ASA. The magnitude of the effect seen depended on the Ca 2+ (endogenous + exogenous) and salicylate concentrations/mg mitochondrial protein, and on the length of the preincubation. Added inorganic phosphate was also required. That salicylate + Ca 2+ induces an increase in the permeability of the mitochondrial inner membrane was demonstrated by the observation that 90% of the intramitochondrial NAD(P) + was released into the surrounding medium upon preincubation of intact mitochondria with these agents. Salicylate + Ca 2+ had virtually no effect on respiration with succinate (+ rotenone) as substrate at salicylate concentrations which markedly affected NAD +-linked substrate oxidation. The presence of rotenone in the preincubation mixture prevented the damaging effects of salicylate + Ca 2+ on the mitochondrial membrane, suggesting that the redox state of intramitochondrial pyridine nucleotides can modulate these effects. The results reported here are similar to those reported previously by our laboratory for the effects of Reye's plasma and allantoin + Ca 2+, and indicate that, like these agents, salicylate and salicyl compounds can potentiate the Ca 2+-induced damage to the mitochondrial inner membrane and may be another factor responsible for Reye's syndrome.