Revolutionizing HIV Treatment: Pioneering Ultra Performance Liquid Chromatography for Emtricitabine, Dolutegravir, and Tenofovir Tablet Validation

Abstract

An uncomplicated and precise method has come into existence for the simultaneous quantification of emtricitabine, dolutegravir, and tenofovir in solid dosage forms. The chromatographic analysis utilized a Hibar100 column (50×2.1 mm, 2 μm) with a mobile phase of 0.1% OPA and acetonitrile in a 60:40 v/v ratio at a constant flow rate of 1.0 mL/min, maintaining a temperature of 30°C. The perfected wavelength at 260.0 nm revealed retention times of 1.951, 1.180, and 1.584 minutes for dolutegravir, emtricitabine, and tenofovir, respectively.