Abstract
Immobilized Artificial Membrane (IAM) chromatography columns have been used to model the in vivo distribution of drug discovery compounds. Regis Technologies Inc., the manufacturer, had to replace the silica support and consequently introduced a new IAM.PC.DD2 column that shows slightly different selectivity towards acidic and basic compounds. The application of the new IAM.PC.DD2 columns has been evaluated and the in vivo distribution models have been compared with the previous batches of columns. It was found that due to the improved endcapping of the silica, some of the positively charged drug molecules showed shorter retention than previously published. Therefore, the column system suitability data have been updated. However, these differences do not significantly affect the previously published models for the volume of distribution, brain tissue binding and drug efficiency. Therefore, the published models can be used with the new IAM.PC.DD2 columns.
Highlights
Pidgeon and Venkataran [1] patented a method for immobilizing phospholipids on HPLC-grade silica stationary phases
The CHI (IAM) values of the test compounds were compared with the CHI (IAM) values obtained on the new batches of Immobilized Artificial Membrane (IAM).PC.DD2 columns
The CHI (IAM) values were compared with the values obtained from the previous batches of IAM columns and it was observed that small polar basic compounds showed significantly shorter retention times on the new IAM.PC.DD2 columns
Summary
Pidgeon and Venkataran [1] patented a method for immobilizing phospholipids on HPLC-grade silica stationary phases. The differences of the models for the estimated brain tissue binding and drug efficiency values of the known drug molecules are investigated and compared with the published data obtained using the previous batches of IAM.PC.DD2 columns.
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