Abstract
The study revisits the taxonomic classification and ecological distribution of Panus velutinus and Panus similis, formerly known as Lentinus velutinus and Lentinus similis, respectively. A total of 45 samples were collected from five distinct conservation areas in Sabah, Malaysia. Detailed morphological analyses and DNA sequencing of the Internal Transcribed Spacer (ITS) region were employed to reassess species identity and distribution. Phylogenetic analyses confirmed the distinct species status of P. velutinus and P. similis, with both species forming separate, well-supported clades (bootstrap support ≥ 70%; posterior probability ≥ 0.9). Notable morphological differences were observed, particularly in basidiospore size, pileus texture, and gleocystidia measurements. Panus velutinus exhibited larger basidiospores (6.5–7 × 3–4 μm) and broader ecological adaptability compared to P. similis (4.5–6 × 2–3 μm), which displayed a more restricted distribution. These findings enhance the taxonomic understanding of these species and highlight the importance of integrating molecular and morphological data to clarify species boundaries within the genus Panus.
Published Version
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