Abstract
Revised Preparation of a Mimetic Tissue Model for Quantitative Imaging Mass Spectrometry
Highlights
Imaging Mass Spectrometry \(IMS) combines spatial information with the highly selective and sensitive nature of mass spectrometry detection to yield a powerful molecular histology technique
The mimetic tissue model represents an approach to IMS quantification which accounts for the major IMS challenges of ion suppression and extraction efficiency through the use of matrix-matched standards
The tissue homogenate used for the mimetic model represents the average ion suppression that would be expected from the corresponding target tissue
Summary
Imaging Mass Spectrometry \(IMS) combines spatial information with the highly selective and sensitive nature of mass spectrometry detection to yield a powerful molecular histology technique. There are several common approaches to building a calibration curve for quantitative IMS proposed in the literature which are outlined nicely by Porta.[7] The first involves spotting the calibration standards directly onto the sample substrate \(typically glass slide) adjacent to the tissue that is to be quantified. While this is a straightforward process, it fails to account for the suppression and extraction efficiency associated with the analyte in the sample tissue. One drawback with this approach had been the elaborate process involved to generate the mimetic tissue model
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