Review the Regulation of Plasma Membrane Calcium Channel in Cancer and Patch Clamp Technique

Nanjun Chen,Qigeng Fang,T Zhu,A Sharifi,M Anpo

doi:10.1051/e3sconf/202127104037

Nanjun Chen, Qigeng Fang + Show 3 more

Open Access

https://doi.org/10.1051/e3sconf/202127104037

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Abstract

As one of the most versatile and universal second messengers, calcium plays an essential role in cell life. Here we briefly reviewed the research progress of how different calcium channels are located at the cell plasma membrane, including voltage-gated calcium channels (VGCCs), receptor-operated channels (ROC), and store-operated channels (ROC). These channels can regulate different cancer progression. Afterward, the patch clamp technique's development and operating principle, an important quantitative method used for ion channel investigation, are introduced in this paper.

Highlights

Calcium (Ca2+), known as a versatile and universal second messenger, is selected by a long course of organic evolution
The uniqueness of Ca2+ signaling is determined by its unique concentration gradient distribution among extracellular, cytosolic, and the endoplasmic reticulum (ER) in resting state
We intend to focus on the latest research advances about Ca2+ signaling regulation in cancer and reveal the mechanism of those regulations as well as patch clamp technique, the most common quantitative detection method for Ca2+

Summary

Introduction

Calcium (Ca2+), known as a versatile and universal second messenger, is selected by a long course of organic evolution It can transduce extracellular signaling and intracellular signaling to regulate numerous enzymes and proteins and further contribute to a wide range of cellular activity. Such universal cellular activities include fertilization, embryonic development, differentiation, transcription factor regulation, apoptosis, autophagy, cancer, and even virus infection [1,2,3,4]. The uniqueness of Ca2+ signaling is determined by its unique concentration gradient distribution among extracellular, cytosolic, and the endoplasmic reticulum (ER) in resting state This feature gives Ca2+ the ability to regulate cellular processes in diverse spatio-temporal patterning, amplitude, and waves [1]. The Voltage-gated calcium channels (VGCCs) are a group of channels mainly permeable to calcium ions in

Voltage-gated Calcium Channels

Patch Clamp Technique the Essential Quantitative Methodology

Conclusion