Abstract
The rapid and direct structural characterization of lipids proves to be critical for studying the functional roles of lipids in many biological processes. Among numerous analytical techniques, ambient ionization mass spectrometry (AIMS) allows for a direct molecular characterization of lipids from various complex biological samples with no/minimal sample pretreatment. Over the recent years, researchers have expanded the applications of the AIMS techniques to lipid structural elucidation via a combination with a series of derivatization strategies (e.g., the Paternò–Büchi (PB) reaction, ozone-induced dissociation (OzID), and epoxidation reaction), including carbon–carbon double bond (C=C) locations and sn-positions isomers. Herein, this review summarizes the reaction mechanisms of various derivatization strategies for C=C bond analysis, typical instrumental setup, and applications of AIMS in the structural elucidation of lipids from various biological samples (e.g., tissues, cells, and biofluids). In addition, future directions of AIMS for lipid structural elucidation are discussed.
Highlights
Lipids are essential biomolecules involved in various cellular functions, including structural components, energy reservoirs, and signal molecules in biological systems [1,2]
Profiling the complete molecular composition of lipidome is helpful for revealing the potential pathways and mechanism in lipid metabolism
Compared with traditional chromatographic separation coupled with Mass spectrometry (MS), ambient ionization mass spectrometry (AIMS) offers simplicity and a higher throughput for lipid structural elucidation
Summary
Lipids are essential biomolecules involved in various cellular functions, including structural components, energy reservoirs, and signal molecules in biological systems [1,2]. Numerous AIMS techniques have been applied for the characterization of C=C locations, as well as sn-positions, in unsaturated lipids via combining with different types of derivatization strategies, including (1) PB reaction, (2) OzID and UVPD, (3) epoxidation reaction and (4) other strategies. Both the mechanism between various derivatization strategies and C=C bond analysis and the typical instrumental setup of AIMS techniques coupled with different derivatization strategies for lipid structural elucidation will be introduced and discussed
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
