Abstract
Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.
Highlights
Elucidating the mechanisms underlying physiological and pathophysiological cellular processes requires the possibility to quantitatively and noninvasively observe cell structure and dynamics down to cellular and subcellular levels.[1]
In a first part of this review, we present the current state of the art of quantitative phase measurement based on digital holographic microscopy (DHM) with a special focus on the advantages provided by the possibility to propagate the whole object wave diffracted by the observed specimen during the numerical reconstruction of the digitally recorded holograms
We describe how quantitative phase (QP)-DHM and quantitative phase tomography (QPT), due to their capacity for quantitatively monitoring cell structure and dynamics through the measurements of different cell biophysical parameters, can begin to address specific questions in the field of cellular neuroscience and psychiatric research
Summary
Elucidating the mechanisms underlying physiological and pathophysiological cellular processes requires the possibility to quantitatively and noninvasively observe cell structure and dynamics down to cellular and subcellular levels.[1]. During the last decades, numerous quantitative phase microscopy (QPM) techniques based on an interferometer or holographic approaches have been developed These approaches are much simpler to implement than classical interference microscopy and provide reliable and quantitative phase mapping of the observed specimen, they have started to be efficiently used in the field of cell biology.[12,13]. In a first part of this review, we present the current state of the art of quantitative phase measurement based on digital holographic microscopy (DHM) with a special focus on the advantages provided by the possibility to propagate the whole object wave (amplitude and phase) diffracted by the observed specimen during the numerical reconstruction of the digitally recorded holograms. Some perspectives related to the development of QPM and/or QPT in high-throughput multimodality approaches are presented
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