Abstract

Low-temperature biopreservation is widely used for the storage of medications. As biochemical reactions in cells are slow at low temperatures, they can be stored for longer periods. However, cryopreservation can result in cryo-injury. Therefore, determining how to achieve long-term biopreservation without causing injury has become an essential research topic. Biological tissues should be stored at different temperature zones depending on the purpose of storage and cell characteristics. In order to achieve better storage efficiency, it is critical to investigate and review the best storage temperature for various biomaterials. The mechanisms of cryo-injury and biopreservation are discussed in this review, and the reasons for the preservation of biological tissues at different temperature zones are also reviewed. Based on the review, the storage characteristics of different temperature ranges are summarized, and the future development of biological storage is prospected. This review has some reference significance for biostorage technology.

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