Abstract
Protein synthesis in embryos and oocytes is irreversibly inhibited when the cells are returned to puromycin-free sea water after previous exposure to puromycin. In unfertilized eggs, on the other hand, it is not irreversibly inhibited. This effect and others are used to show that mature unfertilized eggs are actively engaged in protein synthesis, and that direct assessment of the relative rate of protein synthesis in mature eggs is not hindered by contamination with a small population of very active cells.
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