Abstract
Abstract Incubation of HeLa cells with dimethylsulfoxide results in a partial or complete inhibition of protein synthesis. At a concentration of 12% dimethylsulfoxide, a complete inhibition of protein synthesis occurs within 3 to 4 min. This inhibition is reversible; on removal of dimethylsulfoxide, cells resume their normal rate of protein synthesis. Inhibition of protein synthesis by dimethylsulfoxide is accompanied by a complete breakdown of polyribosomes and release of nascent polypeptides. The breakdown of polyribosomes can be prevented by preincubating cells either with cycloheximide or anisomycin. These studies suggest that dimethylsulfoxide may be a useful tool to synchronize chain initiation in cultured cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
