Reversible immobilization of BSA on Cu-chelated PAMAM dendrimer modified iron oxide nanoparticles

Abstract

• Fe 3 O 4 nanoparticles were successfully grafted by PAMAM dendrimers. • BSA were reversibly immobilized on PAMAM@Fe 3 O 4 nanoparticles. • PAMAM@Fe 3 O 4 nanoparticles are easily recycled by the magnetic field. • The effect of the immobilization strongly depends on the immobilization protocol and experimental conditions. In this study, polyamidoamine (PAMAM) dendrimer coated superparamagnetite nanoparticles were synthesized by growing of PAMAM on amino-silane coated iron oxide nanoparticles. The PAMAM modified superparamagnetite nanoparticles were used as reversible protein immobilization host materials. During the reversible immobilization studies the effect of different metal ions such as; Cu +2 , Zn +2 , Co +2 , Ni +2 on immobilization efficiency of BSA were evaluated. The maximum BSA adsorption capacity of the PAMAM-MNP- Cu +2 beads was observed to be 52.84 mg/g (BSA/PAMAM-MNP) at pH 7.0. Various characteristics of immobilized BSA such as; effect of generation, effect of pH, BSA concentration, temperature, salt concentration and reusability of PAMAM-MNP were evaluated.