Abstract
Lysolecithin prevented the natural cell fusion in monolayer cultures of rat leg muscle cells when it was added in the range of 120 to 160 μg/ml of complete growth medium. The cells rapidly recovered the ability to fuse after withdrawal of the lysolecithin medium and replacement by the regular growth medium. Lysolecithin prevented myogenic cell fusion within a narrow concentration range in the presence of sufficient concentration of calcium and pH values between 7.4 and 7.8. Comparisons between effects of lysolecithin, high cyclic-AMP and low calcium-containing media on cultures of muscle cells were also made. The elaboration of long, thin processes from the bipolar ends of myogenic cells was a feature particularly noticeable with lysolecithin and cyclic-AMP-containing culture plates. The possible influences of other, non-specified, natural surfactants, reacting with lipoprotein micelles of the plasmalemma and subsequently affecting the orientation and arrangement of cell boundaries during differentiation of various cell types, are discussed.
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