Reversible DNA Immobilization on the Neutravidin Coated Surfaces

Abstract

We present a novel approach for single DNA molecule Here we present a novel approach of elongation and reversible immobilization of DNA molecules on neutravidin surfaces by simple control of chemical environments. We demonstrate biocompatibility on the neutravidin surface with restriction enzyme reactions for optical mapping and ligase reactions for tethered DNA. Compared to previous approaches, the use of neutravidin surfaces has unique advantages. First, protein coated surfaces provide biocompatible environments favorable for enzyme reactions. Second, DNA is reversibly immobilized on neutravidin surfaces, which can be beneficial for the development of more complex and integrated genome analysis systems. Finally, neutravidin surfaces are able to tether DNA using biotin labels, which provides a variety of possibilities for the development of advanced genome analysis platforms.