Abstract
Summary Phosphoenolpyruvate carboxylase (EC 4.1.1.31), in dilute leaf extracts from Cynodon dactylon (L.) Pers., is inactivated during incubation at 0°–25 °C. The rate and extent of inactivation are increased at lower temperatures. The inactivation at 0 °C is partially reversible upon transfer to 25 °C and more than fully reversible when glucose-6-phosphate (10 mM) is also added. The activating effect of glucose-6phosphate becomes faster and more pronounced as the temperature is increased. Inactivation is favored by high pH, dilution and high ionic strength. Dissociation of the active tetrameric form into smaller subunits is most probably the cause of inactivation.
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