Reversibility of furazolidone-induced cardiotoxicosis in ducklings

Abstract

Summary Furazolidone cardiotoxicosis was induced in 2 groups (fz and fz-cr groups) of newly hatched male Pekin ducklings (100/group) by feeding a ration containing 650 mg of furazolidone/kg of feed (ppm) for 28 days. A third group (control ration, cr group; n = 100) was fed the same ration without furazolidone. On day 28, the control ration was initiated for the fz-cr group initially given the furazolidone-containing ration, to allow recovery from the effects of the drug, whereas ducklings of the fz group continued to consume the furazolidone-containing ration. Biweekly, beginning with week 4, ducklings were euthanatized to assess severity of gross lesions and to obtain sections of myocardium for histologic and ultrastructural examination. Clinical evidence (increased weight gain, increased feed consumption, decreased mortality, reduced prevalence of palpable ascites) of regression of cardiotoxicosis of ducklings of the fz-cr group was nearly complete by day 56 (28 days after cessation of furazolidone intake). Likewise, regression of gross lesions, as measured by overall prevalence of gross lesions, left ventricular volume, and ascites prevalence and severity, were also essentially complete by day 56. Myofibrillar lysis was not seen in sections from the heart (examined ultrastructurally) obtained from ducklings of the cr group that were euthanatized on day 28, 56, or 98. Myofibrillar lysis was detected in all ducklings (4/4) fed furazolidone (fz and fz-cr groups) and euthanatized on day 28. Myofibrillar lysis was not seen in the heart of ducklings of the fz-cr group that were euthanatized on day 56 or 98. Myofibrillar lysis was detected in the heart from all ducklings of the fz group that were euthanatized on day 56. Leptomeres were observed in cardiac myocytes of ducklings that had been fed furazolidone, but not in those fed only the control ration. Our clinical, gross pathologic, and ultrastructural findings indicate that regression of the cardiac lesions of furazolidone toxicosis may be essentially complete by 28 days after cessation of furazolidone intake. Our ultrastructural findings indicate that furazolidone consumption may result in cardiac dilatation by altering myofibrillar/cytoskeletal attachments of myocytes.