Reversed-phase liquid chromatographic resolution of amino acid enantiomers by derivatization with 2,3,4,6-tetra-O-acetyl-β- d-glucopyranosyl isothiocyanate

Abstract

A novel method for reversed-phase high-performance liquid chromatographic resolution of amino acid enantiomers by the formation of diastereomers using a new chiral reagent, 2,3,46-tetra-O-acetyl-β- d-glucopyranosyl isothiocyanate (GITC), is described. GITC reacts readily with enantiomeric amino acids at room temperature and the reaction mixture can directly be injected into the chromatograph. The derivatives were detected spectrophotometrically at 250 nm. Complete resolutions were observed for all enantiomers examined o a reversed-phase column eluted with aqueous methanol.