Abstract
Relationships between R M values and mobile phase composition have been examined for six fibrate-type drugs — bezafibrate, ciprofibrate, clofibrate, clofibric acid, fenofibrate, and gemfibrozil. They were separated in horizontal chambers on RP18 plates by use of mobile phases containing phosphate buffer and different amounts of six modifiers — acetone, acetonitrile, dioxane, isopropanol, methanol, and tetrahydrofuran. Plates were visualized under UV irradiation at λ = 254 nm, and scanned with a densitometer in multi-wavelength scan mode. Optimum modifier concentrations were also investigated on RP8 and CN plates for comparison. The linearity of relationships between R M and modifier volume fraction, molar fraction, and the logarithm of the molar fraction was calculated. Most results fitted the Snyder-Soczewinski equation with r > 0.95; for almost half r > 0.99. Separation of all the drugs was achieved on RP18 plates with mobile phase containing 70% dioxane in pH 7.60 phosphate buffer.
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