Reversed phase thin layer chromatographic methods for separation of cytokinins

Abstract

Silica gel layers were impregnated with a range of hydrophobic products. Only two such materials, liquid paraffin and the silicone fluid dimethylpolysiloxane, yielded thin layers which separated cytokinins effectively by reversed phase chromatography. The chromatographic behaviour of numerous cytokinins on these layers using a number of solvents is reported. The use of the layers and of integrated normal phase-reversed phase thin layer chromatographic systems to separate cytokinins in crude extracts is discussed.