Reversed-phase stepwise gradient thin-layer chromatography of 19 test dye mixtures with a single void volume of the mobile phase

Abstract

Satisfactory separation of a test mixture of 19 dyes with a general elution problem was obtained by reversed-phase stepwise gradient thin-layer chromatography with single void volume of the mobile phase. An effect of the supplementary mobile phase flux to the surface of the adsorbent layer, observed previously, was eliminated. This improves the flow profile of the mobile phase and the shape of spots, and increases the repeatability of the results within the same plate and between studies. The experimental values of the relative zone/spot position, Rpg, showed good correlation with the results predicted by the computing calculation.