Abstract
Uroshiol in the sap of lac tree, Rhus vernicifera, is directyly into more than ten components by reversed-phase liquid chromatography using acetonitrile—water—acetic acid (80:20:2 of 90:10:2) as the eluent. The 1H NMR parameters, especially those for the triolefinic components, are presented. New substances, 3-(10′ Z, 13′ E, 15′ Z-heptadecatrienyl)catechol and 4-(8′ Z, 11′ E, 13′ z-pentadecatrienyl)catechol, were found, although they were contaminated with 3-(10′ Z, 13′ Z, 16′-heptadecatrienyl)catechol and 4-(8′ Z, 11′ Z 14′-pentadecatrienyl)catechol, respectively. The quantitation by this separation system with refractive index dection nearly coincides with the weight content of each component. Samples from two different sources, Hupei and Kiangsi in China, were analysed by this method.
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