Reversed-phase liquid chromatographic analysis of o-phthaldialdehyde-derivatized free aminoacids in two types of goldfish muscles

Abstract

o-Phthaldialdehyde-derivatized free amino-acids from muscle tissues of goldfish ( Carassius auratus L.) were determined using reversed-phase liquid chromatography (3-μm Ultrasphere ODS) with gradient elution and UV detection of the analyte. An off-line, pre-column derivatization procedure was applied. A full analysis of the derivatives was completed within 40 min. The method showed good selectivity, sensitivity and reproducibility. The developed procedure was employed to quantify free amino-acids in white and red muscle of non-fasted, normoxic goldfish. The differences in the amounts of the individual amino-acids in white and red muscle are given. The rôle of two of the quantitatively most important free amino-acids in white and red muscle of goldfish, histidine and taurine is briefly discussed.