Reversed-phase high-performance thin-layer chromatographic quantification of mimosine from whole plant ofMimosa pudicaLinn

Abstract

A sensitive, simple, and reliable reversed-phase HPTLC method has been established for quantification of mimosine in Mimosa pudica Linn. whole plant powder. The plant powder was first extracted with methanol. The residue was then extracted with water and the aqueous extract was used for quantification. Chromatography was performed on silica gel RP-18 F254s plates with ethyl acetate-glacial acetic acid-water, 6 + 1 + 1.7 ( v/v ), as mobile phase. Quantification was achieved by densitometric scanning at λ max = 282 nm in reflectance-absorbance mode. The response to mimosine was a linear function of concentration over the range 30 to 100 μg mL −1 in the extract. The amount of mimosine in M. pudica was found to be 20 mg g −1 whole plant powder. The method was validated for linearity, precision, accuracy, and robustness.