Abstract
The separation of mono- and diiodoinsulins has been performed using various C 18 columns (LiChrosorb and Vydac), organic modifiers (acetonitrile, 2-propanol and ethanol) and trialkylammonium buffers at various pH values. One system (LiChosorb—2-propanol—triethylammonium formate, pH 6.0) allows complete separation between unlabelled insulin, monoiodoinsulins and diiodoinsulins. The more- or-less reduced binding affinity of the reversed-phase high-performance liquid chromatographic purified tracers is most likely caused by column bleeding.
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