Abstract
The alternative expression of fibronectin mRNA in submandibular salivary glands was analysed using three groups of polymerase chain reaction probes to amplify the cell-binding domain (CBD), and the extra domain (ED)-A and ED-B exon regions in five normal human adults. The fibronectin CBD region was fairly well expressed in the salivary gland while less than trace amounts of ED-A and ED-B exons were expressed. Immunohistochemical staining with an antihuman plasma fibronectin monoclonal antibody, which recognized specifically the CBD region, demonstrated definite positive cytoplasmic staining in the duct-cell area. On the other hand, an anticellular fibronectin ED-A-specific monoclonal antibody was very low positive in duct cells. The results suggest that salivary fibronectin is synthesized in the submandibular salivary gland only, and does not include the ED-A segment. Furthermore, duct cells also produced fibronectin without ED-A.
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