Abstract
Summary Previous molecular operational taxonomic unit (MOTU) survey work with diplogastrid dauers from the abdominal glands of adult female soil-dwelling andrenid bees in Turkey had suggested commensal relationships between species of the genus Andrena and a new species of Koerneria and several species from an unidentified nematode clade near “Mononchoides” (based upon comparisons with GenBank at the time). We used reverse taxonomy on dauer nematodes from Andrena optata from Turkey to successfully culture, morphotype, and sequence adult nematodes that fully matched Pristionchus maupasi and one of the MOTUs previously isolated as dauers from the abdominal glands of A. limata and A. flavipes, and previously designated as belonging to the clade near “Mononchoides.” This study demonstrates the value of reverse taxonomy for resolving MOTU identification issues as the depth of the reference sequence database increases and successful cultures or environmental samples of adults are made available for morphotypic ...
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