Abstract
Abstract: Reverse phase high‐performance liquid chromatographic analyses of nonhydroxy‐and hydroxycerebrosides and sulfatides, as well as ceramides, have been developed. Cerebrosides, ceramides and sulfatides were perbenzoylated then desulfated before chromatographic analysis. These sphingolipid derivatives can be separated according to their fatty‐acid composition by using an ODS 5‐μ, column (reverse phase column containing octadecylsiloxane derivative of silica, of 5‐μm particle size) and solvent systems consisting of various proportions of acetonitrile and methanol. The retention time of an individual homolog appears to be unaffected by the 4‐trans double bond of the sphingosine moiety. These methods allow us to determine fatty‐acid compositions of ceramides, cerebrosides and sulfatides without hydrolysis. Homolog composition of cerebrosides in peripheral nerve from adrenoleukodystrophy patients was investigated by using reverse phase high‐performance liquid chromatography and was found not to be significantly different from control nerves.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
