Abstract
Most of the recent studies in biotechnology have focused on upstream developments such as genetic engineering and bioreactor design. In contrast, downstream processing, which is a major part of production costs, has received disproportionately little attention. Development of techniques that can be scaled up into feasible downstream technology is needed. Selective solubilization of proteins and other biomolecules in reverse micellar organic phase is a promising technique that has the potential to be developed into a liquid-liquid extraction technology for bioseparation. This review describes the concept of ‘enzymes in reverse micelles’ and discusses the ways in which various parameters govern protein solubilization in reverse micelles. Also, a general rationale is provided for developing a process for bioseparation by exploiting the reverse micelle phenomenon.
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